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Ovided a very important tool to facilitate biological study of macrophages [20-23]. Several murine macrophage cell lines from bone marrow [24,25], spleen [26,27], fetal liver [28,29], and lung [30] have been successfully obtained by in vitro infection of primary cell cultures with a recombinant J2 retrovirus carrying the v-raf and v-myc oncogenes. In addition, investigation of the function of both
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
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Allele primers, amplifies a 434 bp DNA fragment from SRA-deficient ZK1, ZK2 and ZK6 cells. With primers for MARCO wild-type allele, amplifies a 500 bp DNA fragment from WT mice; with primers for MARCO mutant allele, amplifies a 850 bp DNA fragment from ZK cells. ZK1, ZK2 and ZK6 clones exhibited both MARCO and SRA-I/II-deficient. PCR products, ca.10 l/each was resolved on a 1.5 agarose gel by gel
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
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Osinic acid (PolyI) and dextran sulfate (DS,Page 3 of(page number not for citation purposes)Particle and Fibre Toxicology 2008, 5:http://www.particleandfibretoxicology.com/content/5/1/WTMS-/-ZKZKModified Wright staining of ZK1 and ZK2 cell lines compared with primary alveolar macrophages Figure 3 Modified Wright staining of ZK1 and ZK2 cell lines compared with primary alveolar macrophages. Primary
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Ing time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. On the average, doubling time of ZK cell lines is between 12 h to 16 h in RPMI complete medium (Fig. 2). Like their parental primary AMs isolated from the MS-/- mice, all of the cell lines are adherent but trypsin-sensitive for passage. Morphology Light microscopic examination of Diff Quik, a modified Wright-
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Tion and analysis, decision to publish or preparation of the manuscript.Tongo et al. Virology Journal 2013, 10:29 http://www.virologyj.com/content/10/1/Page 7 of17. Montavon C, Vergne L, Bourgeois A, Mpoudi-Ngole E, Malonga-Mouellet G, Butel C, Toure-Kane C, Delaporte E, Peeters M: Identification of a new circulating recombinant form of HIV type 1, CRF11-cpx, involving subtypes A, G, J, and CRF01-

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