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Ing time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. On the average, doubling time of ZK cell lines is between 12 h to 16 h in RPMI complete medium (Fig. 2). Like their parental primary AMs isolated from the MS-/- mice, all of the cell lines are adherent but trypsin-sensitive for passage. Morphology Light microscopic examination of Diff Quik, a modified Wright-
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Tion and analysis, decision to publish or preparation of the manuscript.Tongo et al. Virology Journal 2013, 10:29 http://www.virologyj.com/content/10/1/Page 7 of17. Montavon C, Vergne L, Bourgeois A, Mpoudi-Ngole E, Malonga-Mouellet G, Butel C, Toure-Kane C, Delaporte E, Peeters M: Identification of a new circulating recombinant form of HIV type 1, CRF11-cpx, involving subtypes A, G, J, and CRF01-
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Zed SRBCs, only rare unopsonized SRBCs appeared bound to ZK1 cells; most cells did not have any unopsonized SRBCs attached. After binding, these unopsonized SRBCs were easily lysed away (Fig. 5A). Approximately 80 of ZK1 cells were positive for FcR-mediated phagocytosis of opsonized SRBCs (Fig. 5B). Similar results were seen in ZK2 and ZK6 clones (data not shown). Decreased binding and phagocytos
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Zed SRBCs, only rare unopsonized SRBCs appeared bound to ZK1 cells; most cells did not have any unopsonized SRBCs attached. After binding, these unopsonized SRBCs were easily lysed away (Fig. 5A). Approximately 80 of ZK1 cells were positive for FcR-mediated phagocytosis of opsonized SRBCs (Fig. 5B). Similar results were seen in ZK2 and ZK6 clones (data not shown). Decreased binding and phagocytos
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Ogeneous cell populations. We showed here that all three of ZK cell lines responded in a manner comparable to that of primary murine alveolar macrophages. Morphologically, they are alveolar macrophage-like with Diff Quik, a modified Wright staining (Fig. 3). They all highly expressed macrophage antigens, F4/80 and CD11b on cell surfaces by immunofluorescent staining and flow cytometry assays (Fig.
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Night at 37 . Unopsonized (as negative control) or preopsonized SRBC were plated on monolayer of ZK1 cells at a ratio of 20:1 and incubated at 37 for 1 h. After removal of free SRBC by medium exchange and lysis by osmotic shock, the cells on the cover glass were fixed and stained with a modified Wright stain, subsequently examined by light microscopy. Panel A, ZK1 cells were unable to ingest unop
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Surface expression of the macrophage-associated differentiation Ags was assessed by direct immunofluorescence (thick lines). ZK1 cells were incubated with FITC-labeled anti-mouse F4/80 or FITC-labeled anti-mouse CD11b. Mouse IgG2a and IgG2b were used as isotype controls (light lines). Staining of cells with FITC-labeled anti-mouse Ig compared to unstained cells detects the of cells expressing su
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D. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractBackground: Alveolar macrophages (AM) avidly bind and ingest unopsonized inhaled particles and bacteria through class

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