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Nd on the abundance of the target protein. If the yolk is not removed manually, then only 1 or 2 embryos (50?00 ) can be loaded per lane on a gel to avoid overloading effects due to yolk protein. This limits the sensitivity for cellular proteins. The deyolking method enabled us to load significantly more embryos and therefore the signal from specific cellular proteins was increased.Figure 3 demon
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E study targeted larval stages 48 or 72 hpf (hours post fertilization), when the yolk to cell mass ratio is already decreased [5], however, without identifying the proteins. Therefore, it remains unclear whether at this stage analysis without deyolking provides satisfactory information about cellular proteins. Thus, the develop-ment of a reliable method to remove the interfering yolk from cells on
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E representation of the sequence (or a very close homolog) in thePage 3 of(page number not for citation purposes)BMC Developmental Biology 2006, 6:http://www.biomedcentral.com/1471-213X/6/searched database. Since the genome sequencing project of zebrafish is still ongoing, sequence coverage in the databases is incomplete. We therefore compared three databases in regard to the success rate in prote
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E study targeted larval stages 48 or 72 hpf (hours post fertilization), when the yolk to cell mass ratio is already decreased [5], however, without identifying the proteins. Therefore, it remains unclear whether at this stage analysis without deyolking provides satisfactory information about cellular proteins. Thus, the develop-ment of a reliable method to remove the interfering yolk from cells on
1
Ase) makes it a resource for identification, as well as preclinical targeting, of novel mediators of glioma invasion. Galectin-1 was identified in this manner, and has proven in vitro and in vivo to be important in the migration and invasion of glioblastoma cells. Previous work suggests an even greater role of galectin-1 in GBM neoangiogenesis, chemo- and radioresistence, and immune privilege. Tar
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Ase) makes it a resource for identification, as well as preclinical targeting, of novel mediators of glioma invasion. Galectin-1 was identified in this manner, and has proven in vitro and in vivo to be important in the migration and invasion of glioblastoma cells. Previous work suggests an even greater role of galectin-1 in GBM neoangiogenesis, chemo- and radioresistence, and immune privilege. Tar
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Action of the tumor [22,36]. Indeed, abrogating galectin-1 expression renders tumor cells more susceptible to temozolamide treatment [22,41]. Finally, galectin-1 induces apoptosis of activated T-cells [42-46], prevents host animals from mounting tumor vaccine-induced immunity [47], and may cooperate with TGF-beta in GBM-induced immunosuppression [48,49]. In sum, galectin-1 expression may inversely
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Rms and degradation products of the predominant yolk protein Vitellogenin were spread over large parts of the gel (three boxes). Vitellogenin was identified by mass spectrometry. B. Embryo at high stage (3 1/3 hpf). The volume of the yolk cell exceeds the volume of the cells constituting the embryo proper. functions as a nutritional source for the developing embryo [6]. Figure 1A demonstrates how

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